Journal: Biology

Article Title: Regulatory T Cells and IFNγ in Mercury-Induced Autoimmunity: Insights from Adoptive Transfer in B10.S Mice

doi: 10.3390/biology15040298

Figure Lengend Snippet: Serum antinucleolar antibodies (ANoA), exhibiting a distinct clumpy nucleolar staining pattern, were assessed via indirect immunofluorescence in mercury-treated B10.S donor mice ( A ). In contrast, no nucleolar staining of HEp-2 cells was observed when using serum from mercury-treated B10.S IFNγ −/− donor mice ( B ). The images depict animals treated with mercury for 4 weeks. Serum samples were incubated with HEp-2 cells, and staining was detected using FITC-conjugated anti-mouse IgG antibodies.

Article Snippet: Thereafter, the slides were incubated with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse antibody against immunoglobulin total IgG 1 , IgG 2a , or IgG 2b , (1:50 dilution; Southern Biotechnology, Birmingham, AL, USA).

Techniques: Staining, Immunofluorescence, Incubation

Journal: Biology

Article Title: Regulatory T Cells and IFNγ in Mercury-Induced Autoimmunity: Insights from Adoptive Transfer in B10.S Mice

doi: 10.3390/biology15040298

Figure Lengend Snippet: ( A ) Titer of serum antinucleolar antibodies (ANoA). Total IgG titers and IgG subclass titers in B10.S mice over 13 weeks of mercury treatment, assessed by indirect immunofluorescence staining of HEp-2 cells. ( B ) The IgG 2a –IgG 1 index of ANoA (see ). Triangles represent mean values from mice that received cells from water-primed donor mice: non-Treg (▼) and Treg (▽) cells. Circles represent mice that received cells from mercury-primed donor mice: non-Treg (●) and Treg (○) cells. Error bars indicate the standard error of the mean (SEM). Comparative statistical analyses of recipients receiving non-Treg or Treg cells originating from water-primed (1) or mercury-primed (2) donor mice were initially performed using the Kruskal–Wallis test, followed by Dunn’s post hoc procedure to identify specific intergroup differences. Upon detection of statistically significant outcomes, targeted pairwise comparisons between the Treg and non-Treg treatment groups were subsequently conducted using the Mann–Whitney U test. Significance levels: *,# p < 0.05; **, ## p < 0.01; ***, ### p < 0.001. symbols #, ##, and ### denote statistically significant differences between groups A and B. Symbols *, **, and *** denote statistically significant differences between groups C and D.

Article Snippet: Thereafter, the slides were incubated with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse antibody against immunoglobulin total IgG 1 , IgG 2a , or IgG 2b , (1:50 dilution; Southern Biotechnology, Birmingham, AL, USA).

Techniques: Immunofluorescence, Staining, MANN-WHITNEY

Journal: Biology

Article Title: Regulatory T Cells and IFNγ in Mercury-Induced Autoimmunity: Insights from Adoptive Transfer in B10.S Mice

doi: 10.3390/biology15040298

Figure Lengend Snippet: Serum IgG 1 and IgG 2a concentrations in mice over a 13-week period of mercury treatment, measured by ELISA. Triangles represent mean values from mice that received cells from water-primed donor mice: non-Treg (▼) and Treg (▽) cells. Circles represent mice that received cells from mercury-primed donor mice: non-Treg (●) and Treg (○) cells. Error bars indicate the standard error of the mean (SEM). Statistical comparisons between recipients of non-Treg and Treg cells derived from water-primed or mercury-primed donor mice were first conducted using the Kruskal–Wallis test, followed by Dunn’s post hoc analysis. When these tests demonstrated statistical significance, pairwise comparisons between the Treg- and non-Treg–treated groups were subsequently performed using the Mann–Whitney U test. Significance levels: **, ## p < 0.01; *** p < 0.001. Symbols **, and *** denote statistically significant differences between groups C and D.

Article Snippet: Thereafter, the slides were incubated with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse antibody against immunoglobulin total IgG 1 , IgG 2a , or IgG 2b , (1:50 dilution; Southern Biotechnology, Birmingham, AL, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, MANN-WHITNEY